Data Set Group2: Hippocampus Illumina (Aug07)
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Contact Information |
Robert Williams
University of Tennessee Health Science Center
71 S Manassas St, Room 405 TSRB
Memphis, TN 38163 USA
Tel. 901 448-7050
rwilliams@uthsc.edu
Website
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Download datasets and supplementary data files |
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Specifics of this Data Set: |
None
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Summary: |
August 07 ILLUMINA Mouse-6 DATA SET RSN: The LXS Hippocampus Illumina Robust Spline Normalization with No Background correction data set provides estimates of mRNA expression in the hippocampus of 75 LXS recombinant inbred strains, the two parental strains, ILS/Ibg and ISS/Ibg (Inbred Long Sleep and Inbred Short Sleep strains from the Institute of Behavioral Genetics), and the two reciprocal F1 strains (ILSXISSF1, ISSXILSF1). All samples are from normal adult control animals raised in a standard laboratory environment. Subsequent data sets will provide estimates of mRNA expression following restraint stress, ethanol treatment, and stress followed by ethanol using many of the same strains (Lu Lu and colleagues).
A total of 240 pooled hippocampal samples were processed using 40 Illumina Sentrix Mouse-6 v 1.0 oligomer microarray BeadArray slides. Twenty-seven Mouse-6 slides and a total of 160 samples passed stringent quality control and error checking. We should note that this is our first experience using the Illumina platform and the initial set of 13 slides were not included. This particular data set was processed using the Illumina "Robust Spline Normalization with No Background Correction" protocol. Values were log2 transformed and the current data range from 6.481 average (very low or no expression) to 24.852 (extremely high).
As a measure of data quality we often count the number of probes that are associated with LOD scores of greater than 10 (LRS > 46). In this Hippocampus Illumina (Aug 07) RSN data set, ### probes have LRS values >46.
In comparison, here are the yields of QTLs with LOD>10 for other closely related data sets:
- 1050 for Hippocampus Illumina (Aug07) LOESS
- 1162 for Hippocampus Illumina (Aug07) LOESS_NB
- 1129 for Hippocampus Illumina (Aug07) QUANT
- 1176 for Hippocampus Illumina (Aug07) QUANT_NB
- NO DATA for Hippocampus Illumina (Aug07) RSN
- NO DATA for Hippocampus Illumina (Aug07) RSN_NB (THIS DATA SET)
- 1183 for Hippocampus Illumina (May 07) RankInv
- 1167 for Hippocampus Illumina (Oct06) Rank
- 1170 for Hippocampus Illumina (Oct06) RankInv
The LRS achieved in the different version of the LXS Hippocampus data for probe ILM103520706 (Disabled 1; Dab1) are as follow
- 338.4 for Hippocampus Illumina (Aug07) LOESS
- 339.8 for Hippocampus Illumina (Aug07) LOESS_NB
- 370.2 for Hippocampus Illumina (Aug07) QUANT
- 363.5 for Hippocampus Illumina (Aug07) QUANT_NB
- 374.8 for Hippocampus Illumina (Aug07) RSN
- 363.0 for Hippocampus Illumina (Aug07) RSN_NB (THIS DATA SET)
- 360.3 for Hippocampus Illumina (May 07) RankInv
- 358.1 for Hippocampus Illumina (Oct06) Rank
- 358.8 for Hippocampus Illumina (Oct06) RankInv
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About the cases used to generate this set of data: |
Quality Control on Sex Labels: Sex of the samples was validated using sex-specific probe set.
Legend: We evaluated whether or not the sex of samples were labeled correctly by measuring the expression of Xist using probe ILM106520068. In this bar chart the expression of Xist is very low in LXS114 and has a low error term. This is because both arrays are male samples rather than 1 male and 1 female sample.
Data Table 1:
This table lists all arrays by order of strain (index) and includes data on tube ID, strain, age, sex, F generation number, number of animals in each sample pool (pool size), slide ID, slide position (A through F), scan date, and scan batch.
index |
tube ID |
strain |
age |
sex |
gener-
ation |
pool
size |
slide
ID |
slide
position |
batch
by slide |
scan
batch |
1 |
R2851H2 |
ILS |
77 |
F |
>100 |
4 |
1523516003 |
B |
1 |
1 |
2 |
R0595H2 |
ILS |
71 |
F |
>100 |
2 |
1523516030 |
B |
4 |
2 |
3 |
R2874H2 |
ILS |
78 |
M |
>100 |
4 |
1523516011 |
B |
2 |
1 |
4 |
R0585H2 |
ILS |
65 |
M |
>100 |
2 |
1523516028 |
A |
3 |
2 |
5 |
R3281H1 |
ILSXISSF1 |
90 |
F |
NA |
3 |
1562224029 |
A |
28 |
13 |
6 |
R2857H2 |
ISS |
75 |
F |
>100 |
4 |
1523516011 |
A |
2 |
1 |
7 |
R0589H2 |
ISS |
73 |
F |
>100 |
2 |
1523516028 |
B |
3 |
2 |
8 |
R2955H2 |
ISS |
53 |
M |
>100 |
3 |
1523516003 |
A |
1 |
1 |
9 |
R0578H2 |
ISS |
67 |
M |
>100 |
2 |
1523516030 |
A |
4 |
2 |
10 |
R3282H1 |
ISSXILSF1 |
97 |
M |
NA |
3 |
1562224029 |
B |
28 |
13 |
11 |
R2013H2 |
LXS10 |
84 |
F |
27 |
2 |
1562224049 |
E |
18 |
9 |
12 |
R1993H2 |
LXS10 |
78 |
M |
27 |
2 |
1562224036 |
E |
17 |
9 |
13 |
R1997H2 |
LXS100 |
70 |
F |
25 |
2 |
1562224049 |
A |
18 |
9 |
14 |
R1983H2 |
LXS100 |
77 |
M |
25 |
2 |
1562224047 |
F |
16 |
8 |
15 |
R2083H2 |
LXS101 |
85 |
F |
28 |
2 |
1562224050 |
B |
19 |
9 |
16 |
R2084H2 |
LXS101 |
85 |
M |
28 |
2 |
1562224050 |
C |
19 |
9 |
17 |
R2186H2 |
LXS102 |
73 |
F |
29 |
2 |
1523516001 |
C |
9 |
5 |
18 |
R2187H2 |
LXS102 |
73 |
M |
29 |
2 |
1562224053 |
C |
20 |
10 |
19 |
R2809H2 |
LXS103 |
72 |
F |
26 |
2 |
1562224034 |
C |
23 |
11 |
20 |
R2854H1 |
LXS103 |
69 |
M |
26 |
2 |
1523516006 |
D |
6 |
3 |
21 |
R2735H2 |
LXS107 |
78 |
F |
26 |
2 |
1523516028 |
D |
3 |
2 |
22 |
R2738H2 |
LXS107 |
78 |
M |
26 |
2 |
1562224054 |
C |
21 |
10 |
23 |
R2840H2 |
LXS110 |
67 |
F |
25 |
2 |
1562224037 |
D |
24 |
11 |
24 |
R2157H2 |
LXS110 |
75 |
M |
27 |
2 |
1523516033 |
E |
8 |
4 |
25 |
R2172H2 |
LXS112 |
72 |
F |
27 |
2 |
1523516001 |
B |
9 |
5 |
26 |
R2841H2 |
LXS112 |
84 |
M |
27 |
2 |
1562224042 |
D |
27 |
12 |
27 |
R2188H2 |
LXS114 |
72 |
F |
28 |
2 |
1562224053 |
D |
20 |
10 |
28 |
R2204H2 |
LXS114 |
72 |
M |
28 |
2 |
1523516001 |
D |
9 |
5 |
29 |
R2168H2 |
LXS115 |
80 |
F |
27 |
2 |
1523516001 |
A |
9 |
5 |
30 |
R2085H2 |
LXS115 |
71 |
M |
27 |
2 |
1562224050 |
D |
19 |
9 |
31 |
R2811H2 |
LXS117 |
72 |
F |
28 |
2 |
1562224034 |
D |
23 |
11 |
32 |
R2212H2 |
LXS117 |
74 |
M |
27 |
2 |
1523516001 |
E |
9 |
5 |
33 |
R1992H2 |
LXS122 |
86 |
F |
26 |
2 |
1562224036 |
D |
17 |
9 |
34 |
R2219H2 |
LXS122 |
72 |
M |
26 |
2 |
1562224054 |
A |
21 |
10 |
35 |
R2876H2 |
LXS123 |
87 |
F |
28 |
2 |
1562224038 |
C |
25 |
11 |
36 |
R2832H1 |
LXS123 |
77 |
M |
27 |
2 |
1523516011 |
E |
2 |
1 |
37 |
R2872H1 |
LXS124 |
83 |
F |
28 |
2 |
1523516006 |
E |
6 |
3 |
38 |
R2871H2 |
LXS124 |
85 |
M |
28 |
2 |
1562224042 |
F |
27 |
12 |
39 |
R1909H2 |
LXS13 |
79 |
F |
25 |
2 |
1523516024 |
D |
13 |
7 |
40 |
R1901H2 |
LXS13 |
81 |
M |
26 |
2 |
1562224041 |
D |
26 |
12 |
41 |
R2023H2 |
LXS14 |
86 |
F |
25 |
2 |
1562224050 |
A |
19 |
9 |
42 |
R1612H2 |
LXS14 |
85 |
M |
26 |
2 |
1523516015 |
A |
10 |
5 |
43 |
R1936H2 |
LXS16 |
81 |
F |
25 |
2 |
1523516024 |
E |
13 |
7 |
44 |
R1912H2 |
LXS16 |
81 |
M |
25 |
2 |
1523516033 |
B |
8 |
4 |
45 |
R1961H2 |
LXS19 |
66 |
F |
26 |
2 |
1523516029 |
F |
14 |
7 |
46 |
R1904H2 |
LXS19 |
66 |
M |
26 |
2 |
1523516024 |
B |
13 |
7 |
47 |
R1883H2 |
LXS2 |
71 |
F |
27 |
2 |
1523516009 |
F |
12 |
6 |
48 |
R2753H2 |
LXS2 |
74 |
M |
28 |
2 |
1523516030 |
E |
4 |
2 |
49 |
R1729H2 |
LXS22 |
72 |
F |
27 |
2 |
1523516032 |
E |
7 |
4 |
50 |
R2743H2 |
LXS22 |
79 |
M |
27 |
2 |
1562224054 |
D |
21 |
10 |
51 |
R1966H2 |
LXS23 |
73 |
F |
26 |
2 |
1562224032 |
D |
15 |
8 |
52 |
R1971H2 |
LXS23 |
73 |
M |
26 |
2 |
1562224047 |
A |
16 |
8 |
53 |
R2795H2 |
LXS24 |
74 |
F |
26 |
2 |
1523516003 |
C |
1 |
1 |
54 |
R1755H2 |
LXS24 |
56 |
M |
25 |
2 |
1523516007 |
A |
11 |
6 |
55 |
R1986H2 |
LXS25 |
75 |
F |
27 |
2 |
1562224036 |
A |
17 |
9 |
56 |
R2006H2 |
LXS25 |
75 |
M |
27 |
2 |
1562224049 |
C |
18 |
9 |
57 |
R2014H2 |
LXS26 |
82 |
F |
25 |
2 |
1562224049 |
F |
18 |
9 |
58 |
R2009H2 |
LXS26 |
82 |
M |
26 |
2 |
1562224049 |
D |
18 |
9 |
59 |
R2824H1 |
LXS28 |
67 |
F |
28 |
2 |
1562224042 |
A |
27 |
12 |
60 |
R1753H1 |
LXS28 |
72 |
M |
27 |
2 |
1562224041 |
F |
26 |
12 |
61 |
R2765H2 |
LXS3 |
63 |
F |
27 |
2 |
1523516005 |
B |
5 |
3 |
62 |
R1898H2 |
LXS3 |
81 |
M |
26 |
2 |
1562224041 |
C |
26 |
12 |
63 |
R2764H2 |
LXS31 |
73 |
F |
29 |
2 |
1562224060 |
C |
22 |
10 |
64 |
R1908H2 |
LXS31 |
80 |
M |
28 |
2 |
1523516024 |
C |
13 |
7 |
65 |
R2758H2 |
LXS32 |
70 |
F |
26 |
2 |
1523516030 |
F |
4 |
2 |
66 |
R1743H1 |
LXS32 |
67 |
M |
25 |
2 |
1562224041 |
E |
26 |
12 |
67 |
R2794H2 |
LXS34 |
75 |
F |
27 |
2 |
1562224034 |
B |
23 |
11 |
68 |
R2870H2 |
LXS34 |
78 |
F |
27 |
2 |
1562224029 |
C |
28 |
13 |
69 |
R2746H2 |
LXS35 |
80 |
F |
22 |
2 |
1523516030 |
C |
4 |
2 |
70 |
R2747H2 |
LXS35 |
81 |
M |
22 |
2 |
1562224060 |
A |
22 |
10 |
71 |
R1968H2 |
LXS36 |
76 |
F |
25 |
2 |
1562224032 |
E |
15 |
8 |
72 |
R1640H2 |
LXS36 |
76 |
M |
25 |
2 |
1523516032 |
C |
7 |
4 |
73 |
R2835H1 |
LXS38 |
67 |
F |
27 |
2 |
1523516011 |
F |
2 |
1 |
74 |
R2842H2 |
LXS38 |
67 |
M |
27 |
2 |
1562224042 |
E |
27 |
12 |
75 |
R2210H2 |
LXS39 |
81 |
F |
28 |
2 |
1562224053 |
E |
20 |
10 |
76 |
R2736H2 |
LXS39 |
75 |
M |
27 |
2 |
1523516028 |
E |
3 |
2 |
77 |
R1978H2 |
LXS41 |
78 |
F |
26 |
2 |
1562224047 |
C |
16 |
8 |
78 |
R1783H2 |
LXS41 |
56 |
M |
26 |
2 |
1523516007 |
D |
11 |
6 |
79 |
R2822H2 |
LXS42 |
66 |
F |
27 |
2 |
1562224034 |
F |
23 |
11 |
80 |
R2769H2 |
LXS42 |
70 |
M |
27 |
2 |
1562224042 |
B |
27 |
12 |
81 |
R1974H2 |
LXS43 |
84 |
F |
26 |
2 |
1562224047 |
B |
16 |
8 |
82 |
R1733H2 |
LXS43 |
72 |
M |
26 |
2 |
1523516015 |
C |
10 |
5 |
83 |
R1756H2 |
LXS46 |
56 |
F |
25 |
2 |
1523516007 |
B |
11 |
6 |
84 |
R1727H2 |
LXS46 |
70 |
M |
25 |
2 |
1523516032 |
D |
7 |
4 |
85 |
R1970H2 |
LXS48 |
72 |
F |
27 |
2 |
1562224032 |
F |
15 |
8 |
86 |
R1981H2 |
LXS48 |
72 |
M |
27 |
2 |
1562224042 |
C |
27 |
12 |
87 |
R1957H2 |
LXS49 |
72 |
F |
25 |
2 |
1523516029 |
C |
14 |
7 |
88 |
R2259H2 |
LXS49 |
72 |
M |
25 |
2 |
1523516028 |
C |
3 |
2 |
89 |
R2836H1 |
LXS5 |
68 |
F |
28 |
2 |
1523516006 |
A |
6 |
3 |
90 |
R2213H2 |
LXS5 |
80 |
M |
27 |
2 |
1562224053 |
F |
20 |
10 |
91 |
R2791H2 |
LXS50 |
68 |
F |
27 |
2 |
1562224034 |
A |
23 |
11 |
92 |
R1789H2 |
LXS50 |
57 |
M |
26 |
2 |
1523516033 |
A |
8 |
4 |
93 |
R1740H2 |
LXS51 |
68 |
F |
27 |
2 |
1523516032 |
F |
7 |
4 |
94 |
R1734H2 |
LXS51 |
68 |
M |
26 |
2 |
1523516015 |
D |
10 |
5 |
95 |
R2786H2 |
LXS52 |
61 |
F |
27 |
2 |
1562224060 |
D |
22 |
10 |
96 |
R2768H2 |
LXS52 |
61 |
M |
27 |
2 |
1523516005 |
C |
5 |
3 |
97 |
R2154H2 |
LXS54 |
70 |
F |
27 |
2 |
1523516033 |
D |
8 |
4 |
98 |
R2155H2 |
LXS54 |
70 |
M |
27 |
2 |
1562224053 |
A |
20 |
10 |
99 |
R1821H2 |
LXS55 |
77 |
F |
25 |
2 |
1523516009 |
C |
12 |
6 |
100 |
R1951H2 |
LXS55 |
74 |
M |
26 |
2 |
1523516024 |
F |
13 |
7 |
101 |
R2789H2 |
LXS56 |
71 |
F |
25 |
2 |
1523516005 |
F |
5 |
3 |
102 |
R2788H2 |
LXS56 |
71 |
M |
25 |
2 |
1562224060 |
F |
22 |
10 |
103 |
R2787H2 |
LXS59 |
66 |
F |
29 |
2 |
1562224060 |
E |
22 |
10 |
104 |
R2785H2 |
LXS59 |
62 |
M |
29 |
2 |
1523516005 |
E |
5 |
3 |
105 |
R1791H2 |
LXS60 |
58 |
F |
27 |
2 |
1562224038 |
D |
25 |
11 |
106 |
R1792H2 |
LXS60 |
64 |
M |
27 |
2 |
1523516007 |
E |
11 |
6 |
107 |
R1796H2 |
LXS62 |
58 |
F |
27 |
2 |
1523516007 |
F |
11 |
6 |
108 |
R1797H2 |
LXS62 |
58 |
M |
27 |
2 |
1562224038 |
E |
25 |
11 |
109 |
R2220H2 |
LXS64 |
71 |
F |
28 |
2 |
1523516001 |
F |
9 |
5 |
110 |
R2221H2 |
LXS64 |
71 |
M |
28 |
2 |
1562224054 |
B |
21 |
10 |
111 |
R1989H2 |
LXS66 |
73 |
F |
26 |
2 |
1562224036 |
B |
17 |
9 |
112 |
R1843H2 |
LXS66 |
78 |
M |
27 |
2 |
1523516009 |
E |
12 |
6 |
113 |
R2820H2 |
LXS68 |
67 |
F |
29 |
2 |
1523516003 |
E |
1 |
1 |
114 |
R2819H2 |
LXS68 |
67 |
M |
29 |
2 |
1562224034 |
E |
23 |
11 |
115 |
R1963H2 |
LXS7 |
78 |
F |
28 |
2 |
1562224032 |
B |
15 |
8 |
116 |
R1964H2 |
LXS7 |
78 |
M |
28 |
2 |
1562224032 |
C |
15 |
8 |
117 |
R2166H2 |
LXS70 |
72 |
F |
27 |
2 |
1523516033 |
F |
8 |
4 |
118 |
R2745H2 |
LXS70 |
71 |
M |
28 |
2 |
1562224054 |
F |
21 |
10 |
119 |
R2848H2 |
LXS72 |
72 |
F |
27 |
2 |
1562224038 |
A |
25 |
11 |
120 |
R1902H2 |
LXS72 |
66 |
M |
27 |
2 |
1523516024 |
A |
13 |
7 |
121 |
R2750H2 |
LXS73 |
81 |
F |
25 |
2 |
1523516030 |
D |
4 |
2 |
122 |
R1835H2 |
LXS73 |
90 |
M |
24 |
2 |
1523516009 |
D |
12 |
6 |
123 |
R1979H2 |
LXS75 |
59 |
F |
27 |
2 |
1562224047 |
D |
16 |
8 |
124 |
R2826H2 |
LXS75 |
72 |
M |
27 |
2 |
1523516003 |
F |
1 |
1 |
125 |
R2142H2 |
LXS76 |
77 |
F |
26 |
2 |
1562224050 |
E |
19 |
9 |
126 |
R1884H2 |
LXS76 |
85 |
M |
26 |
2 |
1562224041 |
A |
26 |
12 |
127 |
R1959H2 |
LXS78 |
69 |
F |
26 |
2 |
1523516029 |
E |
14 |
7 |
128 |
R1958H2 |
LXS78 |
69 |
M |
26 |
2 |
1523516029 |
D |
14 |
7 |
129 |
R2845H1 |
LXS8 |
70 |
F |
28 |
2 |
1523516006 |
C |
6 |
3 |
130 |
R2156H2 |
LXS8 |
76 |
M |
27 |
2 |
1562224053 |
B |
20 |
10 |
131 |
R1955H2 |
LXS80 |
71 |
F |
25 |
2 |
1523516029 |
A |
14 |
7 |
132 |
R1956H2 |
LXS80 |
71 |
M |
25 |
2 |
1523516029 |
B |
14 |
7 |
133 |
R2830H1 |
LXS84 |
66 |
F |
26 |
2 |
1523516011 |
D |
2 |
1 |
134 |
R2829H2 |
LXS84 |
66 |
M |
26 |
2 |
1562224037 |
A |
24 |
11 |
135 |
R2839H2 |
LXS86 |
68 |
F |
27 |
2 |
1562224037 |
C |
24 |
11 |
136 |
R2838H1 |
LXS86 |
68 |
M |
27 |
2 |
1523516006 |
B |
6 |
3 |
137 |
R2882H1 |
LXS87 |
66 |
F |
27 |
2 |
1523516006 |
F |
6 |
3 |
138 |
R2744H2 |
LXS87 |
71 |
M |
26 |
2 |
1562224054 |
E |
21 |
10 |
139 |
R2831H2 |
LXS88 |
69 |
F |
27 |
2 |
1562224037 |
B |
24 |
11 |
140 |
R2762H2 |
LXS88 |
71 |
M |
27 |
2 |
1523516005 |
A |
5 |
3 |
141 |
R2828H1 |
LXS89 |
75 |
F |
26 |
2 |
1523516011 |
C |
2 |
1 |
142 |
R1962H2 |
LXS89 |
73 |
M |
25 |
2 |
1562224032 |
A |
15 |
8 |
143 |
R1746H2 |
LXS9 |
66 |
F |
26 |
2 |
1523516015 |
F |
10 |
5 |
144 |
R2801H2 |
LXS9 |
68 |
M |
27 |
2 |
1523516003 |
D |
1 |
1 |
145 |
R1812H2 |
LXS90 |
61 |
F |
25 |
2 |
1562224038 |
F |
25 |
11 |
146 |
R1813H2 |
LXS90 |
61 |
M |
25 |
2 |
1523516009 |
A |
12 |
6 |
147 |
R1736H2 |
LXS92 |
66 |
F |
23 |
2 |
1523516015 |
E |
10 |
5 |
148 |
R1609H2 |
LXS92 |
87 |
M |
23 |
2 |
1523516032 |
A |
7 |
4 |
149 |
R1624H2 |
LXS93 |
74 |
F |
26 |
2 |
1523516032 |
B |
7 |
4 |
150 |
R1815H2 |
LXS93 |
61 |
M |
26 |
2 |
1523516009 |
B |
12 |
6 |
151 |
R1991H2 |
LXS94 |
70 |
F |
25 |
2 |
1562224036 |
C |
17 |
9 |
152 |
R2002H2 |
LXS94 |
70 |
M |
25 |
2 |
1562224049 |
B |
18 |
9 |
153 |
R1996H2 |
LXS96 |
75 |
F |
23 |
2 |
1562224036 |
F |
17 |
9 |
154 |
R1772H2 |
LXS96 |
63 |
M |
23 |
2 |
1523516007 |
C |
11 |
6 |
155 |
R2759H2 |
LXS97 |
73 |
F |
26 |
2 |
1562224060 |
B |
22 |
10 |
156 |
R2739H2 |
LXS97 |
79 |
M |
26 |
2 |
1523516028 |
F |
3 |
2 |
157 |
R2149H2 |
LXS98 |
78 |
F |
26 |
2 |
1523516033 |
C |
8 |
4 |
158 |
R1888H2 |
LXS98 |
76 |
M |
26 |
2 |
1562224041 |
B |
26 |
12 |
159 |
R1644H2 |
LXS99 |
79 |
F |
26 |
2 |
1523516015 |
B |
10 |
5 |
160 |
R2145H2 |
LXS99 |
77 |
M |
27 |
2 |
1562224050 |
F |
19 |
9 |
|
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Downloading all data:
All data links (right-most column above) will be available as soon as the global analysis of these data has been accepted for publication. Please see text on Data Sharing Policies, and Conditions and Limitations, and Contacts. Following publication, download a summary text file or Excel file of data. Please contact Dr. Lu Lu if you have any questions on the use of these open data.
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About the tissue used to generate this set of data: |
About the animals and tissue used to generate this set of data:
All animals were raised at the IBG by Bennett and colleagues in an SPF facility. No cases were MHV positive. Mice were killed by cervical dislocation. Whole brain dissections were performed at the IBG by Bennett and colleagues and shipped in RNAlater to Lu Lu and colleagues at UTHSC. Most hippocampal dissections (all were bilateral) were performed by Zhiping Jia. Cerebella, olfactory bulbs, and brain stems were also dissected and stored at -80 deg C using further use. Hippocampal samples are very close to complete (see Lu et al., 2001 but probably include variable amounts of fimbria and choroid plexus (see expression of transthyretin, Ttr, as a marker of choroid plexus).
A pool of dissected tissue from four hippocampi taken from two naive adults of the same strain, sex, and age was collected in one session and used to generate RNA samples. The great majority (75%) of animals were sacrificed between 9:30 AM and 11:30 AM. All animals were sacrificed between 9 AM and 5 PM during the light phase. All RNA samples were extracted at UTHSC by Zhiping Jia.
All animals used in this study were between 53 and 90 days of age (average of 72 days; see Table 1 below).
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About the array platform: |
About the array platform:
Illumina Sentrix Mouse-6 BeadArray Platform: The Mouse6 array consists of 46,116 unique probe sequences, each 50 nucleotides in length, that have been arrayed on glass slides using a novel bead technology.
Dunning M, Smith M, Thorne N, Tavare S (2006) beadarray: An R package to analyse Illumina BeadArrays. R News (the Newsletter of the P Project) 6:17-23. (see pages 17-23 of http://CRAN.R-project.org/doc/Rnews/Rnews_2006-5.pdf).
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About data values and data processing: |
About data processing:
This data set uses the LOESS with Variance Stabilizing Transform (VST) and Background correction from the lumi package downloaded from Bioconductor (http://www.bioconductor.org/). For the more detailed information, please see the lumi package documentation.
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Notes: |
ANNOTATION: In spring of 2007, Robert W. Williams and Hongqiang Li reannotated the Illumina Mouse-6 array content. This new annotation is now incorporated into GeneNetwork. For 46166 probes on the Mouse 6 array platform (including control probes) we have identified 35975 NCBI Entrez Gene IDs; 26481 matched human Gene IDs; 23899 matched rat Gene IDs; 26883 NCBI HomoloGene IDs; and 12791 OMIM IDs.
Position data for the 50-mer Illumina Mouse-6 array were initially downloaded from Sanger at http://www.sanger.ac.uk/Users/avc/Illumina/Mouse-6_V1.gff.gz but we then updated all positions by BLAT analysis from mm6 positions to mm8 positions (Hongqiang Li).
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Experiment Type: |
Experimental Design and Batch Structure: This data set consists arrays processed in 13 groups over a five month period (July 2006 to Dec 2006). Most groups consisted of 12 samples. All arrays in this data set were processed using a single protocol by a single operator, Feng Yiao. Processing was supervised directly by Dr. Lu Lu. All samples were scanned on a single Illumina Beadstation housed in the Hamilton Eye Institute between July 28 and Dec 21, 2006. Details on sample assignment to slides and batches is provide in the table below.
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Contributor: |
About this text file:
INFO file prepared by Xusheng Wang, Oct 24, 2007.
Data set uploaded by Arthur Centeno, Aug 30, 2007.
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Citation: |
Downloading all data:
All data links (right-most column above) will be available as soon as the global analysis of these data has been accepted for publication. Please see text on Data Sharing Policies, and Conditions and Limitations, and Contacts. Following publication, download a summary text file or Excel file of data. Please contact Dr. Lu Lu if you have any questions on the use of these open data.
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Data source acknowledgment: |
Data source acknowledgment:
Data were generated with funds to Lu Lu, Beth Bennett, Mike Miles, Melloni Cook from INIA. · Lu Lu, M.D. Grant Support: NIH U01AA13499, U24AA13513 (Lu Lu, PI)
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Study Id: |
37
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Web services initiated January, 1994 as Portable Dictionary of the Mouse Genome; June 15, 2001 as WebQTL; and Jan 5, 2005 as GeneNetwork.
This site is currently operated by
Rob Williams,
Pjotr Prins,
Zachary Sloan,
Arthur Centeno. Design and code by Pjotr Prins, Zach Sloan, Arthur Centeno, Danny Arends, Christian Fischer, Sam Ockman, Lei Yan, Xiaodong Zhou, Christian Fernandez, Ning Liu, Rudi Alberts, Elissa Chesler, Sujoy Roy, Evan G. Williams, Alexander G. Williams, Kenneth Manly, Jintao Wang, and Robert W. Williams, colleagues.
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GeneNetwork support from:
- The UT Center for Integrative and Translational Genomics
- NIGMS Systems Genetics and Precision Medicine project (R01 GM123489, 2017-2021)
- NIDA NIDA Core Center of Excellence in Transcriptomics, Systems Genetics,
and the Addictome (P30 DA044223, 2017-2022)
- NIA Translational Systems Genetics of Mitochondria, Metabolism, and Aging (R01AG043930, 2013-2018)
- NIAAA Integrative Neuroscience Initiative on Alcoholism (U01 AA016662, U01 AA013499, U24 AA013513, U01 AA014425, 2006-2017)
- NIDA, NIMH, and NIAAA (P20-DA 21131, 2001-2012)
- NCI MMHCC (U01CA105417), NCRR, BIRN, (U24 RR021760)
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