Data Set Group2: JAX Liver HF Affy M430 2.0 (Jul11) MDP
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Contact Information |
Gary Churchill
The Jackson Laboratory
600 Main Street
Bar Harbor, ME 4609 USA
Tel. 207-288-6189
gary.churchill@jax.org
Website
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Download datasets and supplementary data files |
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Specifics of this Data Set: |
None
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Summary: |
High-fat diets are associated with increased obesity and metabolic disease in mice and humans. Here we used analysis of variance (ANOVA) to scrutinize a microarray data set consisting of 10 inbred strains of mice from both sexes fed atherogenic high-fat and control chow diets. An overall F-test was applied to the 40 unique groups of strain-diet-sex to identify 15,288 genes with altered transcription. Bootstrapping k-means clustering separated these changes into four strain-dependent expression patterns, including two sex-related profiles and two diet-related profiles. Sex-induced effects correspond to secretion (males) or fat and energy metabolism (females), whereas diet-induced changes relate to neurological processes (chow) or immune response (high-fat). The full set of pairwise contrasts for differences between strains within sex (90 different statistical tests) uncovered 32,379 total changes. These differences were unevenly distributed across strains and between sexes, indicating that strain-specific responses to high-fat diet differ between sexes. Correlations between expression levels and 8 obesity-related traits identified 5,274 associations between transcript abundance and measured phenotypic endpoints. From this number, 2,678 genes are positively correlated with total cholesterol levels and associate with immune-related categories while 2,596 genes are negatively correlated with cholesterol and connect to cholesterol synthesis. Keywords: gene expression analysis, strain comparision, effect of dietary fat, sex-specific effects
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About the cases used to generate this set of data: |
Index |
Sample ID |
Strain ID |
HF=high-fat (30% fat)
6C=low-fat (6% fat) |
Replicate Animal |
1 |
GSM264767 |
129S1/SvImJ |
6C |
Rep1 |
2 |
GSM264768 |
129S1/SvImJ |
6C |
Rep2 |
3 |
GSM264769 |
129S1/SvImJ |
6C |
Rep3 |
4 |
GSM264770 |
129S1/SvImJ |
HF |
Rep1 |
5 |
GSM264771 |
129S1/SvImJ |
HF |
Rep2 |
6 |
GSM264772 |
129S1/SvImJ |
HF |
Rep3 |
7 |
GSM264773 |
129S1/SvImJ |
6C |
Rep1 |
8 |
GSM264774 |
129S1/SvImJ |
6C |
Rep2 |
9 |
GSM264775 |
129S1/SvImJ |
6C |
Rep3 |
10 |
GSM264776 |
129S1/SvImJ |
HF |
Rep1 |
11 |
GSM264777 |
129S1/SvImJ |
HF |
Rep2 |
12 |
GSM264778 |
129S1/SvImJ |
HF |
Rep3 |
13 |
GSM264779 |
A/J |
6C |
Rep1 |
14 |
GSM264780 |
A/J |
6C |
Rep2 |
15 |
GSM264781 |
A/J |
6C |
Rep3 |
16 |
GSM264782 |
A/J |
HF |
Rep1 |
17 |
GSM264783 |
A/J |
HF |
Rep2 |
18 |
GSM264784 |
A/J |
HF |
Rep3 |
19 |
GSM264785 |
A/J |
6C |
Rep1 |
20 |
GSM264786 |
A/J |
6C |
Rep2 |
21 |
GSM264787 |
A/J |
6C |
Rep3 |
22 |
GSM264788 |
A/J |
HF |
Rep1 |
23 |
GSM264789 |
A/J |
HF |
Rep2 |
24 |
GSM264790 |
A/J |
HF |
Rep3 |
25 |
GSM264791 |
C57BL/6J |
6C |
Rep1 |
26 |
GSM264792 |
C57BL/6J |
6C |
Rep2 |
27 |
GSM264793 |
C57BL/6J |
6C |
Rep3 |
28 |
GSM264794 |
C57BL/6J |
HF |
Rep1 |
29 |
GSM264795 |
C57BL/6J |
HF |
Rep2 |
30 |
GSM264796 |
C57BL/6J |
HF |
Rep3 |
31 |
GSM264797 |
C57BL/6J |
6C |
Rep1 |
32 |
GSM264798 |
C57BL/6J |
6C |
Rep2 |
33 |
GSM264799 |
C57BL/6J |
6C |
Rep3 |
34 |
GSM264800 |
C57BL/6J |
HF |
Rep1 |
35 |
GSM264801 |
C57BL/6J |
HF |
Rep2 |
36 |
GSM264802 |
C57BL/6J |
HF |
Rep3 |
37 |
GSM264803 |
BALB/cJ |
6C |
Rep1 |
38 |
GSM264804 |
BALB/cJ |
6C |
Rep2 |
39 |
GSM264805 |
BALB/cJ |
6C |
Rep3 |
40 |
GSM264806 |
BALB/cJ |
HF |
Rep1 |
41 |
GSM264807 |
BALB/cJ |
HF |
Rep2 |
42 |
GSM264808 |
BALB/cJ |
HF |
Rep3 |
43 |
GSM264809 |
BALB/cJ |
6C |
Rep1 |
44 |
GSM264810 |
BALB/cJ |
6C |
Rep2 |
45 |
GSM264811 |
BALB/cJ |
6C |
Rep3 |
46 |
GSM264813 |
BALB/cJ |
HF |
Rep1 |
47 |
GSM264814 |
BALB/cJ |
HF |
Rep2 |
48 |
GSM264815 |
BALB/cJ |
HF |
Rep3 |
49 |
GSM264845 |
C3H/HeJ |
6C |
Rep1 |
50 |
GSM264846 |
C3H/HeJ |
6C |
Rep2 |
51 |
GSM264847 |
C3H/HeJ |
6C |
Rep3 |
52 |
GSM264848 |
C3H/HeJ |
HF |
Rep1 |
53 |
GSM264849 |
C3H/HeJ |
HF |
Rep2 |
54 |
GSM264850 |
C3H/HeJ |
HF |
Rep3 |
55 |
GSM264852 |
C3H/HeJ |
6C |
Rep1 |
56 |
GSM264853 |
C3H/HeJ |
6C |
Rep2 |
57 |
GSM264855 |
C3H/HeJ |
6C |
Rep3 |
58 |
GSM264856 |
C3H/HeJ |
HF |
Rep1 |
59 |
GSM264857 |
C3H/HeJ |
HF |
Rep2 |
60 |
GSM264858 |
C3H/HeJ |
HF |
Rep3 |
61 |
GSM264859 |
CAST/EiJ |
6C |
Rep1 |
62 |
GSM264861 |
CAST/EiJ |
6C |
Rep2 |
63 |
GSM264862 |
CAST/EiJ |
6C |
Rep3 |
64 |
GSM264863 |
CAST/EiJ |
HF |
Rep1 |
65 |
GSM264864 |
CAST/EiJ |
HF |
Rep2 |
66 |
GSM264865 |
CAST/EiJ |
HF |
Rep3 |
67 |
GSM264866 |
CAST/EiJ |
6C |
Rep1 |
68 |
GSM264867 |
CAST/EiJ |
6C |
Rep2 |
69 |
GSM264868 |
CAST/EiJ |
6C |
Rep3 |
70 |
GSM264869 |
CAST/EiJ |
HF |
Rep1 |
71 |
GSM264870 |
CAST/EiJ |
HF |
Rep2 |
72 |
GSM264871 |
CAST/EiJ |
HF |
Rep3 |
73 |
GSM264872 |
DBA/2J |
6C |
Rep1 |
74 |
GSM264873 |
DBA/2J |
6C |
Rep2 |
75 |
GSM264874 |
DBA/2J |
6C |
Rep3 |
76 |
GSM264875 |
DBA/2J |
HF |
Rep1 |
77 |
GSM264876 |
DBA/2J |
HF |
Rep2 |
78 |
GSM264877 |
DBA/2J |
HF |
Rep3 |
79 |
GSM264890 |
DBA/2J |
6C |
Rep1 |
80 |
GSM264891 |
DBA/2J |
6C |
Rep2 |
81 |
GSM264892 |
DBA/2J |
6C |
Rep3 |
82 |
GSM264893 |
DBA/2J |
HF |
Rep1 |
83 |
GSM264894 |
DBA/2J |
HF |
Rep2 |
84 |
GSM264895 |
DBA/2J |
HF |
Rep3 |
85 |
GSM264896 |
I/LnJ |
6C |
Rep1 |
86 |
GSM264897 |
I/LnJ |
6C |
Rep2 |
87 |
GSM264898 |
I/LnJ |
6C |
Rep3 |
88 |
GSM264899 |
I/LnJ |
HF |
Rep1 |
89 |
GSM264900 |
I/LnJ |
HF |
Rep2 |
90 |
GSM264901 |
I/LnJ |
HF |
Rep3 |
91 |
GSM264902 |
I/LnJ |
6C |
Rep1 |
92 |
GSM264903 |
I/LnJ |
6C |
Rep2 |
93 |
GSM264904 |
I/LnJ |
6C |
Rep3 |
94 |
GSM264905 |
I/LnJ |
HF |
Rep1 |
95 |
GSM264906 |
I/LnJ |
HF |
Rep2 |
96 |
GSM264907 |
I/LnJ |
HF |
Rep3 |
97 |
GSM264908 |
MRL/MpJ-Fas/J |
6C |
Rep1 |
98 |
GSM264909 |
MRL/MpJ-Fas/J |
6C |
Rep2 |
99 |
GSM264910 |
MRL/MpJ-Fas/J |
6C |
Rep3 |
100 |
GSM264912 |
MRL/MpJ-Fas/J |
HF |
Rep1 |
101 |
GSM264913 |
MRL/MpJ-Fas/J |
HF |
Rep2 |
102 |
GSM264914 |
MRL/MpJ-Fas/J |
HF |
Rep3 |
103 |
GSM264915 |
MRL/MpJ-Fas/J |
6C |
Rep1 |
104 |
GSM264916 |
MRL/MpJ-Fas/J |
6C |
Rep2 |
105 |
GSM264917 |
MRL/MpJ-Fas/J |
6C |
Rep3 |
106 |
GSM264918 |
MRL/MpJ-Fas/J |
HF |
Rep1 |
107 |
GSM264920 |
MRL/MpJ-Fas/J |
HF |
Rep2 |
108 |
GSM264921 |
MRL/MpJ-Fas/J |
HF |
Rep3 |
109 |
GSM264922 |
NZB/BlNJ |
6C |
Rep1 |
110 |
GSM264924 |
NZB/BlNJ |
6C |
Rep2 |
111 |
GSM264925 |
NZB/BlNJ |
6C |
Rep3 |
112 |
GSM264926 |
NZB/BlNJ |
HF |
Rep1 |
113 |
GSM264928 |
NZB/BlNJ |
HF |
Rep2 |
114 |
GSM264929 |
NZB/BlNJ |
HF |
Rep3 |
115 |
GSM264930 |
NZB/BlNJ |
6C |
Rep1 |
116 |
GSM264931 |
NZB/BlNJ |
6C |
Rep2 |
117 |
GSM264932 |
NZB/BlNJ |
6C |
Rep3 |
118 |
GSM264933 |
NZB/BlNJ |
HF |
Rep1 |
119 |
GSM264935 |
NZB/BlNJ |
HF |
Rep2 |
120 |
GSM264936 |
NZB/BlNJ |
HF |
Rep3 |
121 |
GSM265061 |
PERA/EiJ |
6C |
Rep1 |
122 |
GSM265062 |
PERA/EiJ |
6C |
Rep2 |
123 |
GSM265063 |
PERA/EiJ |
6C |
Rep3 |
124 |
GSM265064 |
PERA/EiJ |
HF |
Rep1 |
125 |
GSM265065 |
PERA/EiJ |
HF |
Rep2 |
126 |
GSM265066 |
PERA/EiJ |
HF |
Rep3 |
127 |
GSM265067 |
PERA/EiJ |
6C |
Rep1 |
128 |
GSM265068 |
PERA/EiJ |
6C |
Rep2 |
129 |
GSM265069 |
PERA/EiJ |
6C |
Rep3 |
130 |
GSM265070 |
PERA/EiJ |
HF |
Rep1 |
131 |
GSM265071 |
PERA/EiJ |
HF |
Rep2 |
132 |
GSM265072 |
PERA/EiJ |
HF |
Rep3 |
133 |
GSM265074 |
SM/J |
6C |
Rep1 |
134 |
GSM265075 |
SM/J |
6C |
Rep2 |
135 |
GSM265105 |
SM/J |
6C |
Rep3 |
136 |
GSM265217 |
SM/J |
HF |
Rep1 |
137 |
GSM265248 |
SM/J |
HF |
Rep2 |
138 |
GSM265275 |
SM/J |
HF |
Rep3 |
139 |
GSM265324 |
SM/J |
6C |
Rep1 |
140 |
GSM265331 |
SM/J |
6C |
Rep2 |
141 |
GSM265357 |
SM/J |
6C |
Rep3 |
142 |
GSM265358 |
SM/J |
HF |
Rep1 |
143 |
GSM265359 |
SM/J |
HF |
Rep2 |
144 |
GSM265360 |
SM/J |
HF |
Rep3 |
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About the tissue used to generate this set of data: |
Liver
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About the array platform: |
[Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array. Affymetrix submissions are typically submitted to GEO using the GEOarchive method described at http://www.ncbi.nlm.nih.gov/projects/geo/info/geo_affy.html All probe sets represented on the GeneChip Mouse Expression Set 430 are included on the GeneChip Mouse Genome 430 2.0 Array. The sequences from which these probe sets were derived were selected from GenBank«, dbEST, and RefSeq. The sequence clusters were created from the UniGene database (Build 107, June 2002) and then refined by analysis and comparison with the publicly available draft assembly of the mouse genome from the Whitehead Institute for Genome Research (MGSC, April 2002).
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About data values and data processing: |
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Notes: |
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Experiment Type: |
Expression profiling by array.
One group of mice was fed an atherogenic high-fat (30% fat) diet containing cholic acid to increase fat uptake and another was fed a low-fat (6% fat) regular chow diet. Males and females from both diets were studied for mouse strains 129S1/SvImJ, A/J, BALB/cJ, C3H/HeJ, C57BL/6J, CAST/EiJ, DBA/2J, I/LnJ, MRL/MpJ-Tnfrsf6lpr/J, NZB/BINJ, PERA/Ei, and SM/J. All strains were sacrificed between 11- and 13 weeks of age except for CAST and PERA, which were harvested after 50 weeks of age. CAST and PERA were subsequently removed from our analysis based on discrepant harvest age, but can be found in our database (see below). Three replicate animals were used for each combination of diet, strain, and sex, resulting in a total of 120 mice surveyed for gene expression.
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Contributor: |
Churchill GA, Paigen B, Shockley KR, Witmer D
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Citation: |
Burgess-Herbert SL, Cox A, Tsaih SW, Paigen B. Practical applications of the bioinformatics toolbox for narrowing quantitative trait loci. Genetics 2008 Dec;180(4):2227-35. PMID: 18845850 Shockley KR, Witmer D, Burgess-Herbert SL, Paigen B et al. Effects of atherogenic diet on hepatic gene expression across mouse strains. Physiol Genomics 2009 Nov 6;39(3):172-82. PMID: 19671657
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Data source acknowledgment: |
Churchill GA, Paigen B, Shockley KR, Witmer D
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Study Id: |
133
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